Topic > Seed Germination - 2362

Introduction: Seed germination is a process in which the quiescent seed is absorbed by water, the seed radical elongates and breaks the seed coat surrounding the embryo. However, most plant seeds do not germinate immediately and undergo a period of dormancy. These dormant seeds are normally dehydrated, contain inactive enzymes, and are resistant to adverse conditions. These characteristics allowed the seed to disperse before germination occurred and to survive adverse environmental conditions such as drought. For a seed to break its dormancy, optimal conditions were needed for the seed to germinate. There are three important conditions for seeds to germinate: adequate temperature, sufficient water supply and also a satisfactory level of oxygen. Water supply is important as it is used to rehydrate seed tissues and induce breakdown of the seed coat which served as a physical barrier for root elongation (Debeaujon et al., 2000). In addition to this, oxygen is also an essential element for seed germination because aerobic respiration of the seed tissues produces energy, ATP, which is necessary for the further development of the embryo. The optimal temperature for potential seed germination varies between plant species, but it is one of the important factors that influence seed germination. Factors such as light and hormones also contribute to the seed germination process. Exposure to light is critical for the germination of some plant seeds, and light has an indirect effect on the surrounding temperature. Hormones are important as they signal the production of enzymes in the seed to carry out processes such as mitotic division, cell wall degradation, and cell differentiation. Therefore, the purpose of this experiment is to study the effect of light, planting depth and hormones on seed germination. Materials and methods: In section A, the effect of light on seed germination was studied. The materials used in section A were 100 lettuce seeds, 100 green beans, 4 Petri dishes, cotton wool and 4 filter papers. 2 Petri dishes were labeled as water + light (LS) and water + dark (LS). The other 2 Petri dishes were labeled water + light (GB) and water + dark (GB). All Petri dishes were placed on filter paper moistened with 2 ml of water. Then, 5 ml of water is used to moisten the cotton ball placed on top of all the Petri dishes. 50 lettuce seeds were placed on each Petri dish labeled LS and 50 green beans were placed on each Petri dish labeled GB.